pcr产物电泳后为何条带过宽,而且有的条带清晰,有的则不清晰

问题描述:

pcr产物电泳后为何条带过宽,而且有的条带清晰,有的则不清晰

You can try either of the following or both:
1.Clean the PCR mix before run it using Qiagen kit,it may be better.
2.heat the PCR mix at 60C for 5 min.
make sure your agarose gel is made correctly.